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Indlela Yokuvimbela Ukuqhekeka Kweshubhu Le-Microcentrifuge Ku-RCF Ephezulu Nokwandisa Ukutholakala Kwe-Biomolecule

Ku-biology yamangqamuzana, i-proteomics, kanye nokulungiselela amasampula we-multi-omics, amashubhu ajwayelekile we-microcentrifuge (1.5 mL / 2.0 mL) avame ukuhlangabezana nezinkinga ezimbili ezibucayi zokwehluleka: ukuqhekeka kodonga lweshubhu noma ukuphuma kwe-cap ngesikhathi sokugibela ngesivinini esikhulu (), kanye nokulahleka kwe-target analyte ngenxa yokubambelela okungaqondile (adsorption) kwe-DNA eningi, i-RNA, noma ama-peptide ezindongeni zeshubhu.
Ukuqhekeka kweshubhu le-microcentrifuge nokuphuma kwesivalo ngesikhathi sokuhlukaniswa ngejubane eliphezulu kubangela ukulahleka kwesampula le-biomolecule

Amaphuzu Abalulekile

  • Kungani Amashubhu Aqhekeka Noma Aqhume:
  • Umsuka Wokuthola Okuphansi:
  • Isixazululo Sobunjiniyela: I-polypropylene emsulwa yezinga lezokwelapha ye-USP Class VI, izivalo ezihlanganisiwe zokuphepha, kanye nokuguqulwa kwendawo yokugcina okuncane kakhulu

Izimbangela Zokwehluleka Kweshubhu Nokulahleka Kwesampula Emisebenzini Ye-Bioworkflows

Umdwebo wezindlela ezimbili zokwehluleka: ukuqhekeka kodonga lweshubhu ngenxa ye-RCF ephezulu kanye nokunamathela okungaqondile kwe-DNA protein ezindongeni zeshubhu
Ukukhishwa Okunzima (i-Phenol/Chloroform) / I-RCF Ephezulu (>20,000×g) ➔ Ingcindezi Yodonga Nokunwebeka Kweplastiki ➔ Ukuqhekeka Kweshubhu / Ukuphuma Kwe-Cap ➔ Ukulahleka Kwe-Biological Matrix
Amaprotheni/ama-DNA asezingeni eliphansi ➔ Ukusebenzisana okungabonakali noma okungathandeki nge-Surface Hydrophobic ➔ Ukunamathela odongeni lwepulasitiki ➔ Umphumela ophansi (Ukutholakala okungaphansi kuka-70%)
  1. Ukusikwa nge-Centrifugal Shear kanye Nokuhlaselwa yi-Solvent:
  2. Ukubophezela Okungaqondile Ebusweni:
  3. Ukungcola Kwe-Additive & Slip-Agent:

I-Matrix Yokwenza: Ama-Tube Ajwayelekile vs. Ama-Tube E-CHANCEPRO Ultra-Low Retention

Ipharamitha Yokusebenza
Ama-Microtubes Ajwayelekile / Okuthengiswayo
Izinga Elijwayelekile Lomkhiqizo Ophezulu (isb., i-Eppendorf Safe-Lock)
Amashubhu E-CHANCEPRO Okugcina Okuncane Kakhulu
Amandla Amakhulu Okugibela (RCF)
​
Kufika ku-
​
(Okuqinisekisiwe)
Ubumsulwa be-Resin
I-PP yezohwebo / ingxube egaywe kabusha
I-Virgin PP (I-USP Class VI)
I-PP yezokwelapha engu-100% ye-Virgin (I-USP Class VI)
Ama-Slip Agents angavuzwayo
Akhona (Ubungozi be-Oleamide/DiHEMDA)
Akukho / Kuqinisekisiwe
100% Akukho Ama-Slip Agents kanye nama-Plasticizers
Ukutholakala kwe-DNA/RNA (
)
60% – 75%
85% – 92%
> 98% (Udonga olungathandeki olushintshwe ngokomzimba)
Ukulahleka kwamaprotheni/ama-peptide
Okuphezulu (>25% ukunamathela odongeni)
Okuphansi
Okuphansi kakhulu (<3% ukubopha)
Ububanzi be-Thermal kanye ne-Cryo
kuya
​
kuya
​
ukuze
(Kungenziwa i-autoclave)
Indlela Yokuvala Ikhava
Ukufinyela okujwayelekile kokushaya
I-mechanical clip/lock
Ungqimba Lokuphepha Lokunemba (Zero Pop-off)

Izinqubo Ezi-4 Ezingcono Kakhulu Zokuvikela Amasampula Ku-RCF Esezingeni Eliphezulu

  1. Qinisekisa Iphrofayela Yesisekelo Se-Tube Ngokuqhathanisa Ne-Rotor Adaptors:
  2. Sebenzisa Ama-Tube Okuphepha Okukhiya Ezinto Eziyingozi:
  3. Gwema Ama-Tube Acwebezeliswe Nge-Silicone Ku-Mass Spectrometry:ukuguqulwa komhlaba ongaphezulu kanye nokuhlanganiswa kwe-resin
  4. Izinqubo Zokwehla Kancane Kancane:

Ama-Microcentrifuge Tubes E-CHANCEPRO Ultra-Low Retention

Kuklanyelwe izinqubo ezidingekayo ze-genomics, proteomics, single-cell, kanye ne-high-speed centrifugation:
I-CHANCEPRO ishubhu le-microcentrifuge eline-ultra-low retention nesivalo esine-safety-lock kanye nendawo ephansi yokugwema amaconsi
  • Isilinganiso Esiphezulu Se-RCF ():
  • Ubuchwepheshe Obuthuthukile Bokunciphisa Ukubamba:
  • Umklamo Wokuvala Okuphephile Nge-Safety-Lock:
  • Ubumsulwa be-Biopurity okuqinisekisiwe:

Thintana nathi

Ukuhlanganisa Ubusha, Ukusebenzisa Izitayela.

Ngokubhalisa incwadi yethu yezindaba, uyavuma neMigomo Yethu Yesevisi kanye neNqubomgomo Yobumfihlo.

Xhumana Nathi